Basic performance and applicable fields of various magnetic beads
Magnetic beads are important equipment used in biological immunology. They have super paramagnetism and can quickly accumulate in a magnetic field. After leaving the magnetic field, they can contribute to the uniform dispersion of magnetic separation. By coating the surface of the magnetic beads with specificity Antibodies, receptors, etc., are used to separate and purify the target in the sample, and have been widely used in many fields such as immunoassay, nucleic acid separation and extraction, and cell sorting. There are many types of magnetic beads, and their basic performance and applicable fields are also different. Next, the editor of Aisen will share with you the basic properties and applicable fields of various magnetic beads.
Basic characteristics of various magnetic beads
1. Basic performance of carboxyl magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) Density of carboxyl group: about 2000 nmol/mg
(4) Surface potential: about -35 mV
(5) Concentration: 10 mg/mL
2. Basic performance of carboxyl magnetic beads (low non-specificity)
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) Density of carboxyl group: about 1000 nmol/mg
(4) Surface potential: about -35 mV
(5) Concentration: 10 mg/mL
3. The basic performance of amino magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) Density of carboxyl group: about 600 nmol/mg
(4) Surface potential: about 30mV
(5) Concentration: 10 mg/mL
4. Basic performance of mycostreptavidin magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) SA load: 60-90μg/mg
(4) Surface potential: about -35mV
(5) Concentration: 10 mg/mL
5. The basic performance of protein A magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) SPA loading capacity: 70-75μg/mg
(4) Surface potential: about -29mV
(5) Concentration: 30 mg/mL
6. The basic performance of protein G magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) SPG load: 70-85μg/mg
(4) Surface potential: about -26mV
(5) Concentration: 30 mg/mL
7. The basic performance of protein A/G magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) SPA/G loading capacity: 70-85μg/mg
(4) Surface potential: about -26mV
(5) Concentration: 30 mg/mL
8. The basic performance of nucleic acid extraction silicon hydroxy magnetic beads
(1) Electron microscope size: about 1μm
(2) Magnetic content: 35%-45%
(3) Surface potential: about -26mV
(4) Concentration: 30 mg/mL
Applicable fields of various magnetic beads
1. Applicable fields of carboxyl magnetic beads
It can be covalently coupled with biological ligands such as peptides, proteins, antibodies, oligonucleotides, etc. under the action of special chemical reagents (such as EDC), and can be used in the following fields: affinity purification, chemiluminescence, cell sorting, biology Sensors, immunoassays, molecular diagnostics, etc.
2. Applicable fields of carboxyl magnetic beads (low non-specificity)
With low non-specific adsorption, it can be covalently coupled with peptides, proteins, antibodies, oligonucleotides and other biological ligands under the action of special chemical reagents (such as EDC), especially suitable for protein/cell sorting and immune monitoring , Biosensing, high-sensitivity analysis, etc.
3. Applicable fields of amino magnetic beads
It can be covalently coupled with biological ligands such as peptides, proteins, oligonucleotides, drug molecules, glycoproteins, etc. under the action of special chemical reagents (such as glutaraldehyde), and can be used as a good basic material for coating. An important carrier tool in medical and molecular biology research.
4. Applicable fields of mycostreptavidin magnetic beads
It can be efficiently coupled with biotinylated peptides, proteins, antibodies, oligonucleotides and other biological ligands, and is used in the following fields: chemiluminescence, cell extraction, cell classification, high-sensitivity analysis, immunoassay, biotin-labeled PCR extraction , Biosensing, gene expression analysis, etc.
5. Applicable fields of protein A magnetic beads
It is widely used in small systems, high-throughput IgG purification and labeling, immunoprecipitation, ChIP and protein separation. This technology is fast and gentle, and has very little physical pressure on the target protein. It is especially suitable for separating and concentrating unstable and easily decomposable components.
6. Applicable fields of protein G magnetic beads
It is widely used in small systems, high-throughput IgG purification and labeling, immunoprecipitation, ChIP and protein separation. This technology is fast and gentle, and has very little physical pressure on the target protein. It is especially suitable for separating and concentrating unstable and easily decomposable components.
7. Applicable fields of protein A/G magnetic beads
It is widely used in small systems, high-throughput IgG purification and labeling, immunoprecipitation, ChIP and protein separation. This technology is fast and gentle, and has very little physical pressure on the target protein. It is especially suitable for separating and concentrating unstable and easily decomposable components. Compared with SPA magnetic beads and SPG magnetic beads, SPA/G magnetic beads are suitable for more types of IgG.
8. Applicable fields of nucleic acid extraction silicon hydroxy magnetic beads
It is suitable for nucleic acid extraction and purification of plants, animals, whole blood, serum free, viruses, forensic samples, plasmids, PCR products, etc. It can be used with automatic nucleic acid extractors for high-throughput and automated operations.
The basic performance and applicable fields of various magnetic beads are described above. It can be seen that there are various types of biomagnetic beads, the basic performance is different, and the field of application is also different. Aisen Biotechnology produces a variety of types of magnetic beads with excellent performance and a wide range of applications. Customers in need are welcome to call for consultation.
