How accurate is the nucleic acid detection?

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Commonly used infectious disease laboratory tests include microbial culture, virus isolation, nucleic acid testing, and antibody testing. Nucleic acid testing is also a method to detect viruses, which can detect infections earlier. Nucleic acid tests have been carried out for hepatitis B, hepatitis C, and AIDS in clinical practice, which can detect the presence of viruses in the blood before antibodies are produced. For some respiratory diseases, nucleic acid testing of throat swabs or respiratory secretions can be used to determine whether they are infected, and whether they are contagious. Seasonal influenza, avian influenza, coronavirus infections, etc., can be easily judged whether there are viral components in the upper respiratory tract through throat swab testing, so as to determine whether the tested person is an infected person. So, how accurate is the nucleic acid detection?

At present, there are no exact large-sample statistics to prove the accuracy of nucleic acid detection, but there are many factors that affect the accuracy, such as the quality of the detection reagents, the time of infection of the tested person (too early or too late will reduce the accuracy), specimens The cooperation of the examinee when taking the material (poor cooperation may cause the specimen not to be taken in place) and the operational proficiency of the examiner, etc.

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If all aspects of nucleic acid detection are accurate, the accuracy of nucleic acid detection can reach more than 95% under ideal conditions. But due to various reasons, false negative results will occur. The so-called false negative means that the testee is indeed an infected person, but the nucleic acid test result is negative, which is a false negative. With the improvement of technology, the accuracy rate of various medical institutions is improving rapidly, and the false negative rate is continuously decreasing.

In addition, for people who have had close contact with confirmed or suspected cases, two nucleic acid tests are usually required to exclude them. For people who have been to high-risk areas of the new coronavirus, if they have fever and respiratory symptoms, a negative nucleic acid test may have a false negative, so two nucleic acid tests are needed to rule out. In addition, if the doctor judges that a nucleic acid test is negative and still cannot be ruled out, the nucleic acid test needs to be tested again. Sometimes the doctor will also recommend chest CT, blood tests, and serum antibody tests for the new coronavirus to determine whether it is infected.

Advantages and uses of common magnetic beads

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High-quality magnetic beads have the characteristics of uniform size distribution, high surface loading, extremely short magnetic response time, high dispersion stability, etc., which can quickly and efficiently separate the test object from the sample, greatly improving the detection efficiency. In addition, there are many types of magnetic beads, and different magnetic beads have different advantages and different uses. Next, the editor of Aisen will share with you the relevant knowledge about the advantages and uses of common magnetic beads.

Advantages and uses of carboxyl magnetic beads

1. Advantages

(1) It can be coupled with biomolecules such as protein, nucleic acid, streptavidin, polypeptide, enzyme, etc. through the activation of EDCNHS;

(2) 1μm in size, uniform size, granular roughness on the surface, high specific surface area, extremely high carboxyl loading capacity, and low non-specific adsorption capacity;

(3) It has superparamagnetism, fast magnetic response speed, good monodispersity, and can ensure the uniformity of the reaction and the consistency of detection;

(4) It has a highly hydrophilic surface and good biocompatibility;

(5) Excellent suspension, slow semi-sedimentation speed, suitable for automatic operation.

2. Purpose

Chemiluminescence, gene capture, protein purification, cell sorting.

Advantages and uses of streptavidin magnetic beads

1. Advantages

(1) It can be used for the coupling of biotinylated proteins, nucleic acids, polysaccharides, lipids, enzymes and other biomolecules immediately;

(2) 1μm in size, uniform size, granular roughness on the surface, high specific surface area, extremely high streptavidin loading and biotinylated protein/nucleic acid coupling rate;

(3) Surface modification of hydrophilic polymer molecules and covalent coupling of multi-layer streptavidin. Streptavidin is densely adsorbed on the surface with few exposed sites on the surface, which greatly reduces non-specific adsorption ；

(4) It has good suspension and is stable within pH (3.0-9.0), and can be used for automated high-throughput experimental operations.

2. Purpose

Chemiluminescence, protein and nucleic acid separation.

Advantages and uses of amino magnetic beads

1. Advantages

(1) It can be coupled with biological molecules such as proteins, nucleic acids, polysaccharides, lipids, enzymes, etc. through a variety of coupling methods;

(2) 1μm in size, uniform size, granular roughness on the surface, high specific surface area, and extremely high amino loading;

(3) It has superparamagnetism, fast magnetic response speed, good monodispersity, and can ensure the uniformity of the reaction and the consistency of detection;

(4) It has a highly hydrophilic surface and good biocompatibility;

(5) Excellent suspension, slow semi-sedimentation speed, suitable for automatic operation.

2. Purpose

Chemiluminescence, cell, virus, nucleic acid, antibody separation and purification.

The above is the introduction of the advantages and uses of common magnetic beads. Different magnetic beads have different advantages and performances, and their uses are also different. The suitable magnetic beads should be selected according to their performance characteristics. The magnetic beads produced by Aisen Biotechnology are rich in varieties and excellent in performance. Customers in need are welcome to call for consultation.

What is the principle of magnetic bead method for nucleic acid extraction

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With the rapid development of genetic diagnosis, genetically modified food detection, personalized medicine, etc., the current nucleic acid extraction technology can no longer meet the needs of today’s biotechnology, and there is an urgent need for a high-throughput and automated nucleic acid extraction method. In this context, the magnetic bead method for nucleic acid extraction came into being.

The use of magnetic beads for DNA extraction is one of the areas where their biological value is maximized. The main types of microspheres used include silanol magnetic beads and oligo magnetic beads. Magnetic beads with surface groups can specifically and reversibly bind to nucleic acids released in the test sample. At the same time, the magnetic response capability of the magnetic beads is used to carry out directional movement and enrichment under the action of an external magnetic field, so as to realize the separation and purification of nucleic acid. The main advantages of the magnetic bead method for nucleic acid extraction include simple and automated operation, large-scale operation, and short time.

1. What is a magnetic bead

Magnetic beads are a kind of special nano-micron materials. Their size is usually between a few tenths of a few microns to a few microns, which is only one tenth to one tenth of the diameter of a human hair, which cannot be observed by the naked eye. Single magnetic bead. They are superparamagnetic, they can move quickly to one side in a magnetic field, gather together, and can quickly return to a dispersed state after removing the magnetic field.

The surface of the magnetic beads used for nucleic acid extraction has specific properties. Under certain conditions, the viral nucleic acid can be adsorbed on the surface, and under other conditions, the viral nucleic acid can be released from the surface for subsequent detection steps.

2.The principle of magnetic bead method of nucleic acid extraction

The binding of nucleic acid to magnetic beads mainly relies on electrostatic, hydrophobic and hydrogen bonding. The DNA/RNA in the cell or tissue is released under the action of the lysis solution. At this time, the surface-modified superparamagnetic silica nanomagnetic beads “specifically bind” with nucleic acid to form a “nucleic acid-magnetic bead complex”. Then under the action of an external magnetic field, the complex is separated. After the eluate is washed to remove the non-specifically adsorbed magazines, desalted, and purified, the nucleic acid substance to be extracted is obtained.

The nucleic acid extraction principle is according to surface of superparamagnetic nanoparticles is modified and modified by nanotechnology to prepare superparamagnetic silica nanomagnetic beads. The magnetic beads can specifically recognize and efficiently bind with nucleic acid molecules on the microscopic interface. Using the superparamagnetism of silica nanospheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, DNA and DNA can be separated from samples such as blood, animal tissues, food, and pathogenic microorganisms. RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiological testing, food safety testing, molecular biology research and other fields.

In fact, since testing institutions need to process a large number of samples every day, most of the nucleic acid extraction steps are carried out by means of an automatic nucleic acid extraction instrument with a magnetic bead method nucleic acid extraction kit.

What are the factors affecting nucleic acid extraction by magnetic beads?

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In the past two years, as pathogen detection has become more popular, pathogen nucleic acid detection has gradually entered people’s field of vision and has become the gold standard for pathogen detection. Faced with large quantities of samples, testing results need to be issued in a short period of time, and people’s needs for fast and efficient testing methods are becoming more and more urgent. As an important step of nucleic acid detection, the use of nucleic acid extraction kits also faces the requirements of throughput and high efficiency.. Automatic nucleic acid extraction (magnetic bead method nucleic acid extraction) has been quickly promoted and applied because of its fast, efficient, easy-to-use, safe and non-toxic characteristics, and has become a standard in laboratories. However, during the extraction process, certain factors will affect the extraction results and require the operator’s attention. The editor of Aisen has compiled the relevant knowledge of the factors affecting nucleic acid extraction by magnetic bead method, and let’s share it with you.

Magnetic bead method nucleic acid extraction consists of lysis, washing and elution steps. The factors that affect these steps will also affect the extraction efficiency of magnetic beads. Below we explain each step one by one.

　　 Factors affecting the lysis step

　　1.Lysis solution composition

For some samples that are difficult to lyse, the lysis ability of the lysate can be enhanced by increasing the concentration of guanidine salt and surfactant. Guanidine salts and surfactants can promote protein denaturation, destroy cell membranes, dissociate nucleic acids and proteins, and release more nucleic acids.

　　2.pyrolysis temperature and time

Increasing the temperature and increasing the lysis time can make the cell lysis more thoroughly and get more nucleic acids. You need to explore the appropriate temperature and time; too high temperature or too long time may also lead to nucleic acid fragmentation or reduced extraction efficiency caused by alcohol volatilization.

　　3.magnetic beads adsorption capacity

magnetic bead extraction series magnetic beads are generally silanol magnetic beads, and the adsorption capacity of the magnetic beads is affected by the specific surface area of ​​the magnetic beads and the density of the modified group. Generally, magnetic beads with more modified groups and larger specific surface area are selected. Generally, the smaller the particle diameter of the magnetic beads, the larger the specific surface area. But it is not that the smaller the particle size of the magnetic beads, the better, the smaller the particle size of the magnetic beads, the adsorption speed will also slow down, you can choose according to the actual sample situation. The binding buffer is generally combined with isopropanol or ethanol. Generally, the binding capacity can be enhanced by increasing the ratio of alcohol, and the concentration of chaotropic salt should be increased to promote the binding of nucleic acid and magnetic beads.

　　 Factors affecting washing steps

Washing is very important for the entire extraction step. It is related to the purity of nucleic acid, which directly affects downstream PCR applications. The oscillation amplitude and mixing time of the magnetic bar will affect the washing efficiency.

　　1.Magnetic rod oscillation amplitude

If the oscillation amplitude of the magnetic rod is too small, there will be impurities remaining on the magnetic beads, which will affect the ability of the magnetic beads to adsorb nucleic acids; if the oscillation is too violent, part of the nucleic acid will be lost or the nucleic acid will be broken or degraded, and the yield will decrease.

　　2.mixing time

If the mixing time is too short, there will be impurities remaining on the magnetic beads, which will affect the ability of the magnetic beads to adsorb nucleic acids; if the mixing time is too long, part of the nucleic acid will be lost or the nucleic acid will be broken or degraded, and the yield will decrease.

COVID-19 Virus Nucleic Acid Extraction Kits & System

　　 The influence of elution step factors

The elution step directly affects the yield of nucleic acid. The factors affecting the elution efficiency are the drying degree of the magnetic beads and the elution temperature and time.

　　 1.Dryness degree of magnetic beads

If the magnetic beads are not fully dried, there may be impurities such as alcohol, which will affect the subsequent PCR reaction; if the magnetic beads are too dry, the nucleic acid may dry up on the magnetic beads, resulting in a decrease in elution efficiency.

　　2.elution temperature and time

Appropriately increasing the elution temperature and time will help the nucleic acid to be released from the magnetic beads. If the time is too long or the temperature is too high, the nucleic acid may be degraded, and the balance needs to be grasped.

The relevant content about the factors that affect the magnetic bead method of nucleic acid extraction is the above. When performing the magnetic bead method of nucleic acid extraction, ensure that the above factors are reasonable, so that the extraction quality can be better. Aisen Biotechnology has extensive experience in the production of nucleic acid extraction systems and nucleic acid extraction kits. It uses high-quality technology and reliable quality. Customers in need are welcome to call for consultation.

Magnetic bead method of nucleic acid extraction steps and principles

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With the continuous development of biomedicine, nucleic acid extraction technology is also being updated. Magnetic bead nucleic acid extraction, as a new type of nucleic acid extraction technology, can extract nucleic acids with high throughput and automation, meeting the needs of today’s biotechnology. In addition, the steps of magnetic bead method for nucleic acid extraction are relatively simple, easy to operate, and are well received. Next, I will share with you the steps and principles of magnetic bead method for nucleic acid extraction.

Magnetic bead method for nucleic acid extraction steps

1. Add 1 mL of deionized water to each bottle of 40mg proteinase K dry powder to a final concentration of 40mg/mL, and add 500 μL of deionized water to each bottle of 20mg proteinase K dry powder to a final concentration of 40mg/mL, and mix it upside down. It can be completely dissolved once, and it can be stored at 4℃ for short-term use. Please keep it at -20℃ for long-term storage. Repeated freezing and thawing should not exceed five times.

2. The tissue sample is ground into fine powder with 10-30 mg of liquid nitrogen, transferred to a 1.5 mL centrifuge tube, resuspended in 200 μL of 1 X PBS buffer, and then proceeded to the next step. The liquid sample goes directly to the next step.

3. Take 500μL of virus lysate VL and add it to a 1.5mL enzyme-free centrifuge tube, then add 25μL of proteinase K and 25μL of viral magnetic beads (shaken well before adding), and then add 200μL of serum/plasma/tissue homogenate sample , Vortex and mix well, 55% C water bath for 20 minutes, invert and mix for 10 seconds every 5 minutes.

4. Take out the enzyme-free centrifuge tube in the water bath, place it on the magnetic stand, and magnetize for 90 seconds to completely adsorb the magnetic beads. Use a pipette to carefully aspirate and discard the supernatant (be careful not to attract the magnetic beads at the bottom of the tube).

5. Remove the centrifuge tube from the magnetic stand, add 700μL of rinsing solution BufferA, mix by pipetting or vortex for 30 seconds to resuspend the magnetic beads, then place the centrifuge tube on the magnetic stand, and magnetize for 90S to make the beads Adsorbed completely, carefully aspirate and discard the supernatant with a pipette.

6. Take 700μL of rinsing solution BufferB, add it to the centrifuge tube, try not to blow off the magnetic beads, and then directly aspirate and discard the supernatant.

7. Add 80μL of elution buffer, remove the centrifuge tube, and bathe in a water bath at 56°C for 5 min. During this period, vortex 3-4 times to elute the nucleic acid from the magnetic beads.

8. Place the centrifuge tube on the magnetic stand and magnetically attract for 60 seconds, transfer the liquid to a new 1.5mL enzyme-free centrifuge tube, and proceed directly to the downstream experiment or store at -20°C.

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The principle of magnetic bead method for nucleic acid extraction

Its principle mainly relies on electrostatic interaction, hydrophobic interaction and hydrogen bonding interaction. The DNA/RNA in the cell or tissue is released under the action of the lysate. At this time, the surface-modified superparamagnetic silica nanomagnetic beads “specifically bind” with nucleic acid to form a “nucleic acid-magnetic bead complex”. Then under the action of an external magnetic field, the complex is separated. Then the eluate is used to wash away non-specifically adsorbed impurities, desalt and purify to obtain the nucleic acid substance to be extracted.

The above is the introduction to the steps and principles of the magnetic bead method for nucleic acid extraction. The magnetic beads and nucleic acid extraction system produced by Aisen Biotechnology have excellent performance and reliable quality. Customers in need are welcome to call for consultation.

What is nano magnetic bead technology?

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Nanomagnetic beads refer to small magnetic particles whose size is suitable to be measured by nanometers, generally 1-100 nanometers. This kind of magnetic beads has a very special magnetic property called superparamagnetism, that is, it has a strong magnetic field in an external magnetic field. Responsiveness, and after the magnetic field is removed, the magnetic properties of the magnetic particles disappear immediately, that is, there is no remanence, and they are uniformly dispersed in the solution again. Traditional DNA separation technology includes precipitation, centrifugation and other processes. These purification methods have complicated steps, time-consuming, low yield, and contact with toxic reagents. It is difficult to realize automatic operation; however, the use of magnetic carrier microsphere separation technology can overcome it well. These shortcomings enable rapid and efficient sample preparation. Therefore, nanomagnetic bead technology is an important direction for the development of DNA purification methods in the future.

What are nano magnetic beads

The so-called nano magnetic beads refer to small magnetic particles whose size is suitable to be measured by nanometers, generally referring to 1-100 nanometers. This kind of magnetic beads has a very special magnetic property called superparamagnetism, that is, it has a strong magnetic field in an external magnetic field. Magnetic responsiveness, and after the magnetic field is removed, the magnetic properties of the magnetic particles disappear immediately, that is, there is no remanence, and they are uniformly dispersed in the solution again.
Scientists favor this feature, which can be used to adsorb a certain component in the liquid, and then magnetically separate the magnetic beads to achieve the purpose of separating the components. Of course, in order to be able to adsorb the desired substance, specific groups must be wrapped on the outside of the particles, such as amino, hydroxyl, carboxyl, sulfhydryl and other functional groups. These groups are specifically combined with the target molecule, and then collected by magnetic force. Magnetic beads can separate the required substances.
The magnetic beads themselves are mostly inorganic materials, the most common material is ferroferric oxide, and the outer covering is basically organic material. According to the position relationship between the magnetic bead itself and the coating material, there are four types of magnetic bead structures, namely, core-shell type, mosaic type, core-shell type, and core-shell type.

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Among them, the first core-shell type has been studied and used the most. Its magnetic material is used as the core, and the polymer material is used as a shell to wrap the outside of the magnetic particles. The inorganic magnetic material at the core endows the entire particle with superparamagnetism. Separation, the high-molecular substance in the shell gives the entire particle high-efficiency adsorption, that is, specificity. In a fully mixed and uniform liquid environment, the polymer shell is combined with the target substance through its functional group, and the movement and collection of the entire particle is controlled by an external magnetic field, and finally the goal of separation of the target substance is achieved.

The use of magnetic beads for DNA extraction is one of the areas where their biological value is maximized. The main types of microspheres used include silanol magnetic beads and oligo magnetic beads. Magnetic beads with surface groups can specifically and reversibly bind to nucleic acids released in the test sample. At the same time, the magnetic response capability of the magnetic beads is used to carry out directional movement and enrichment under the action of an external magnetic field, so as to realize the separation and purification of nucleic acids. The main advantages of the magnetic bead method for nucleic acid extraction include simple and automated operation, large-scale operation, and short time.

Six Misunderstandings in the Magnetic Bead Method of Nucleic Acid Extraction

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The use of biological magnetic beads to extract nucleic acid is a novel method of nucleic acid extraction. Compared with the traditional chloroform, isoamyl alcohol extraction method and spin column kit method, this method is still understood by many people. There are also some misunderstandings in the process of purification of nucleic acids by the pearl method.

Misunderstanding 1. The more magnetic beads, the better the extraction effect

Some people think that when the extraction effect is not good, increase the amount of magnetic beads. They think that adding a little more magnetic beads can attract more nucleic acids. In fact, this idea is not advisable.
The main feature of magnetic beads is that they can be dispersed in a liquid or separated from a liquid phase in a solid state under the action of an external magnetic field. For any reagent system, the ratio of magnetic beads to liquid should have a certain value, exceeding a certain ratio Excessive magnetic beads will lose their dispersion characteristics because they cannot be uniformly dispersed in the liquid, and the efficiency of contact between the nucleic acid magnetic beads and the liquid cannot be fully increased during the washing process.
Excessive magnetic beads will also adsorb more impurities, which has a great influence on the effect of impurity removal. Even sometimes, too many magnetic beads will adsorb protease, lysozyme and other functional components that play a major role in the liquid system, resulting in low efficiency of the entire kit. In many cases, when the extraction effect is not good, reducing the amount of magnetic beads used is the best way to improve the extraction effect.
Normally, the amount of reference magnetic beads given by the magnetic bead method kit is slightly excessive. Therefore, it is not often necessary to increase the amount of magnetic beads to improve the adsorption efficiency. However, if it is determined that the amount of magnetic beads is insufficient, the extraction effect is not good. Well, it is possible to improve the extraction effect by increasing the amount of magnetic beads within a certain range.

Misunderstanding 2. The more reagents used, the better the extraction effect

However, for the magnetic bead method, each increase in the volume of a part of the liquid reduces the probability of more magnetic bead collisions, and reducing the probability of magnetic bead collisions will result in a significant drop in the adsorption rate. Therefore, in many cases, although adding lysis solution and washing solution can indeed enhance lysis and enhance washing, the core of magnetic bead extraction is the efficiency of magnetic bead adsorption of nucleic acids. The efficiency of magnetic bead collision cannot be guaranteed, but the efficiency of nucleic acid extraction cannot be guaranteed. Yes, so simply increasing the amount of reagents used to improve the extraction effect may not be completely effective.

Misunderstanding 3. The more washing times, the better the extraction effect

When there are too many impurities in the extracted nucleic acid, the user will consider washing several times to obtain a purer nucleic acid. Increasing the number of washes is indeed conducive to the purification of nucleic acids, but considering that each wash will lose a certain amount of nucleic acid and increase the possibility of nucleic acid fragmentation and hydrolysis, it is generally appropriate to control the number of washes at 2 to 4 times.

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Misunderstanding 4. The more samples are used, the better the extraction effect

When the sample is not fresh enough or the nucleic acid content itself is low, the nucleic acid extraction effect is often not good, and many teachers will use multiple samples to increase the amount of nucleic acid extraction.
However, simply increasing the sample sampling volume may sometimes introduce too many impurities, exceeding the lysing capacity of the lysate, and also reduce the extraction efficiency. Therefore, it is not recommended to simply increase the sample sampling volume to achieve the purpose of increasing the extraction volume.
If the extraction volume is indeed too low due to insufficient sample volume, it is recommended to go through the enrichment or concentration step before starting the extraction. Or increasing the completeness of lysis and exposing more nucleic acids is also a solution.

Misunderstanding 5. If a certain kind of magnetic bead is good, it should be effective in all tests

There are many types of magnetic beads, different particle size, different dispersion, different magnetic response time, different coating base matrix, different outer modified functional group, different coating density, different functional group arm length, which will lead to magnetic beads Features vary greatly.
Therefore, the experiments and systems that different magnetic beads adapt to are also different. Just like the reagents for nucleic acid extraction, the formulas are not exactly the same, and the properties of the magnetic beads that are also used for nucleic acid extraction are not exactly the same.
Some magnetic beads show higher adsorption efficiency in the extraction of constant nucleic acid, and some magnetic beads are more suitable for the extraction of trace nucleic acid. Some magnetic beads are suitable for more acidic reagent systems, and some magnetic beads are suitable for more alkaline reagent systems. Some magnetic beads have good magnetic responsiveness but fast settling speed, which is more suitable for magnetic rod type automatic extractor; some magnetic beads have slow settling speed but long magnetic response time, and are more suitable for pipette type automatic extractor.
There is rarely a kind of magnetic beads that can be applied to all experimental situations. Except for the fixed kit, in most cases, the magnetic beads and the reagent system need to be adjusted for a certain period of time.

Misunderstanding 6. It is not good to compare with a certain kit, that is, the magnetic beads are not good

In the process of screening magnetic beads, many customers simply replace the magnetic beads with the same amount under the mature reagent system to compare the effects of magnetic beads.
In this way, it is easy to conclude that certain magnetic beads are not effective, but in fact, because different magnetic beads are suitable for different systems and dosages, they often need to be adjusted to obtain better extraction results.

What are the types of magnetic bead nucleic acid extraction kits?

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The magnetic bead method of nucleic acid extraction technology was produced in the 1980s, and now there are mature kits and formed into an industry. The magnetic bead method nucleic acid extraction kit is a high-tech product that integrates nanotechnology, molecular biology technology, biomedical technology and forensic technology. It can be widely used in the fields of molecular biology, such as genome research, molecular evolution research, genetic disease research in medicine, mutation gene detection, tumor screening, HPV detection, transplant matching, blood spots in forensic medicine samples, etc. With the wide application of magnetic bead nucleic acid, no matter in the field of virus, bacteria or eukaryotic nucleic acid extraction, there are corresponding magnetic bead nucleic acid extraction kits available for use. The following editor of Aisen will take stock of magnetic beads for everyone. What are the types of plasmid extraction kits for magnetic beads and nucleic acid extraction kits?

　　 Magnetic bead method virus DNA/RNA extraction kit

Magnetic Bead Method Viral DNA/RNA Extraction Kit can separate and purify high-quality viral DNA/RNA from serum, plasma, lymph, cell-free body fluid, cell culture supernatant, urine or various virus preservation solutions. The extracted viral DNA/RNA has high yield, high purity, stable and reliable quality, and the extracted product can be used for downstream molecular experiments such as reverse transcription, PCR, RT-PCR, fluorescent quantitative PCR, etc. The operation process of this kind of kit is simple and fast, does not need phenol, chloroform and other toxic organic solvent extraction, and does not need time-consuming steps of centrifugation and precipitation.

　　 Magnetic Bead Method Bacterial Genomic DNA Extraction Kit

Bacterial Genomic DNA Extraction Kit is not only suitable for the extraction of genomic DNA from Gram-negative bacteria, but also for the extraction of genomic DNA from Gram-positive bacteria. The kit uses the principle of superparamagnetic particles to adsorb nucleic acids under specific conditions to purify DNA. The purified genomic DNA is suitable for a variety of subsequent experiments, such as PCR, restriction digestion, and gene sequencing. The operation process of the test type kit is simple and fast, does not need phenol, chloroform and other toxic organic solvent extraction, and does not need time-consuming steps of centrifugation and precipitation.

　　 Magnetic Bead Method Plasmid Extraction Kit

Plasmid extraction means to remove RNA, separate the plasmid from the bacterial genomic DNA, remove proteins and other impurities, to obtain a relatively pure plasmid. The special coated magnetic beads have a strong affinity to the target DNA under certain conditions, and when the conditions change, the magnetic beads release the adsorbed DNA, which can achieve the purpose of rapid isolation and purification of DNA, and can separate and purify high-quality plasmids from samples DNA. The magnetic bead method plasmid extraction kit has the characteristics of not involving toxic reagents, safety, convenience, and high purity of the extracted DNA. The extracted plasmid can be used in various downstream molecular biology experiments.

　　 Magnetic Bead Method Whole Blood Genomic DNA Extraction Kit

Magnetic Bead Method Whole Blood DNA Extraction Kit uses special coated biological magnetic beads to separate and purify high-quality nucleic acids from whole blood through a unique separation buffer system. The kit provides a simple, fast, and efficient method for extracting blood DNA, which is suitable for extracting blood DNA from fresh or frozen anticoagulated blood (blood samples treated with citrate, EDTA, and heparin). The nucleic acid purified by the magnetic bead method whole blood DNA extraction kit can be applied to various normal-scale operations, including restriction enzyme digestion, PCR library establishment, molecular labeling, southern hybridization test.

　　 magnetic bead method plasma free DNA extraction kit

Due to the low DNA content and small DNA fragments, plasma samples have higher requirements for extraction, and product testing often requires PCR amplification to complete. The plasma free magnetic bead DNA extraction kit has outstanding performance in terms of sensitivity and stability. The operation process of this kind of kit is simple and fast, does not require extraction with toxic organic solvents such as phenol and chloroform, and does not require time-consuming steps of centrifugation and precipitation.

　　 Magnetic bead method animal tissue genomic DNA extraction kit

Magnetic bead method animal tissue genomic DNA extraction kit is suitable for the extraction of genomic DNA from animal tissues. It has the characteristics of less time-consuming, simple operation, safety and non-toxicity, and stable effect.

　　 Magnetic Bead Method Plant Genomic DNA Extraction Kit

Magnetic Bead Method Plant Genomic DNA Extraction Kit uses magnetic nano-separation technology to extract genomic DNA from plant tissues. The extraction process is simple and fast, and the entire extraction process is safe and non-toxic. It is suitable for automatic nucleic acid extraction platform. The extracted genomic DNA is of high purity and can be applied to various downstream molecular biology experiments.

　　 Magnetic bead method PCR product purification kit

The magnetic bead method PCR product purification kit is suitable for high-throughput and rapid recovery of nucleic acid fragments from various PCR products or enzymatic reaction solutions, and can effectively remove oligonucleotides and primer dimerization in PCR products or enzymatic reaction solutions Body, salt, protein and other pollution. The purified product can be directly used for sequencing, enzyme digestion, hybridization, PCR and other experiments. The system is easy to operate and can be used in conjunction with most nucleic acid purifiers, and the entire operation process can be fully automated.

The general introduction of the types of magnetic bead nucleic acid extraction kits is as described above. It can be seen that different nucleic acid extraction kits have different uses. Aisen Technology produces a variety of magnetic bead nucleic acid extraction kits. Customers in need are welcome Call for consultation.

What are the main components of the nucleic acid extraction kit?

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Nucleic Acid Extraction Kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in nucleic acid extraction and purification technology. With the rapid development of genetic diagnosis, genetically modified food detection, personalized medicine, etc., the current nucleic acid extraction technology can no longer meet the needs of today’s biotechnology, and there is an urgent need for a high-throughput and automated nucleic acid extraction method. In this context, a nucleic acid extraction kit (magnetic bead method) came into being. Next, the editor of Aisen will introduce to you what are the main components of the nucleic acid extraction kit.

　　1, sodium lauryl sulfate

Sodium Lauryl Sulfate is an organic matter, white or light yellow powder, soluble in water, insensitive to alkali and hard water. It has decontamination, emulsification and excellent foaming power. It is an anionic surfactant that is slightly toxic to the human body, and its biodegradability is >90%.

It can break the hydrogen bonds in and between molecules, unfold the molecules, destroy the secondary and tertiary structure of protein molecules, denature the protein, and destroy the binding of protein and nucleic acid at high temperature, and promote the release of nucleic acid.

　　2, ethyl phenyl polyethylene glycol

Ethyl Phenyl Polyethylene Glycol is abbreviated as NP-40, which is a very mild non-ionic detergent, often used in cell lysis, protein treatment, histochemical treatment, hybrid washing, etc. 1% concentration can basically destroy the cell membrane, but the damage to the nuclear membrane is weak.

　　3, weak cation chelating resin

Weak cation chelating resin is a chemical chelating resin composed of styrene and divinylbenzene copolymer, containing pairs of iminodiacetate ions, which can chelate multivalent ions, especially for high-valent metal ions. Very high affinity and chelation. Under the conditions of low ionic strength, alkalinity and boiling, the cell membrane can be ruptured and the protein can be denatured. Chelex particles are removed by centrifugation, and the bound material is separated from the DNA.

　　4, proteinase K

is a powerful proteolytic enzyme isolated from Candida albicans. It has a high specific activity and is a key reagent for DNA extraction. In DNA extraction, the main function is to enzymatically decompose histones bound to nucleic acids, so that the DNA is freed in the solution.

　　5, polyethylene glycol octyl phenyl ether

Polyethylene glycol octyl phenyl ether is abbreviated as Triton X-100. It is a non-ionic surfactant. It does not dissociate in water, has high stability in solution, and is not easily affected by strong electrolyte inorganic salts. Combines with lipids such as phospholipids in biological membranes to form soluble complexes; the hydrophobic end can also combine with the hydrophobic regions of membrane proteins to form complexes and dissolve in solution.

　　 6, guanidine isothiocyanate

Guanidine isothiocyanate is a powerful protein denaturant, which can quickly dissolve protein and cause cell structure to be broken. Nucleoprotein is quickly separated from nucleic acid due to the destruction and disappearance of its secondary structure.

　　 7, ethylenediaminetetraacetic acid

Ethylenediaminetetraacetic acid is an organic compound, which is a white powder under normal temperature and pressure. It is a chelating agent that can bind to divalent metal ions. Since most nucleases and some proteases require divalent metal ions, they are often used as inhibitors of nucleases and proteases; they can also be used to remove the inhibitory effect of heavy metal ions on enzymes.

The above is the introduction to the main components of the nucleic acid extraction kit. The nucleic acid extraction kit uses magnetic beads to adsorb DNA/RNA to achieve the purpose of rapid DNA/RNA purification, and the above-mentioned chemical components play an important role in this process. The magnetic bead method nucleic acid extraction kit designed and produced by Aisen Biotechnology is reliable in quality, convenient to use and excellent in effect. Customers in need are welcome to call for consultation.

What is the detection principle of the rapid nucleic acid detection kit?

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The kit is to configure the various raw materials that need to be used in PCR replication and amplification in advance, and then the medical staff can greatly save the configuration time when making the diagnosis. At the same time, the conditions (temperature, time, etc.) used by the kit also need to be explored and explored by the developer. Continuous optimization, testing and verification are required to ensure the accuracy and precision of the kit and avoid misdiagnosis and missed diagnosis. In the case of (false positive and false negative), a standardized novel coronavirus nucleic acid detection kit for clinical diagnosis can be obtained subsequently.

1. What is the rapid detection of new coronavirus nucleic acid?

As we all know, rapid nucleic acid testing is currently the “gold standard” for the detection of new coronaviruses, and it is also a direct evidence for judging whether there is a new coronavirus in the subject. The new coronavirus is an RNA virus, and the specific RNA sequence in the virus is a marker that distinguishes the virus from other pathogens. After the emergence of the new type of coronavirus, Chinese scientists completed the analysis of the complete genome sequence of the new type of coronavirus in a very short time, and discovered the specific nucleic acid sequence in the new type of coronavirus. If the specific nucleic acid sequence of the new coronavirus is detected in the patient’s sample, it indicates that the patient may be infected by the new coronavirus.

 

 

2. Principles of COVID-19 rapid detection kit

Most of the rapid nucleic acid detection kits use the fluorescent quantitative PCR method. The genetic material of organisms is divided into two types, DNA and RNA. DNA has a double helix-like structure, which is more stable, while RNA is a single-stranded structure. Coronavirus is an RNA virus, which is easier to mutate and adapt to the human body. The most important way to confirm whether a patient is infected with the new coronavirus is to detect whether the genetic material of the virus is present in the patient or suspected case.

However, the amount of RNA that can be extracted in patient samples is limited, so a method is needed to quickly detect the nucleic acid content in virus samples. Fluorescence quantitative PCR is a new type of quantitative test technology launched by an American company in 1996. It uses fluorescent dyes or fluorescently-labeled specific probes to label and track PCR products for real-time monitoring of the reaction. So, what is PCR? PCR is the polymerase chain reaction, which can greatly increase the trace amount of DNA. The process of fluorescent quantitative PCR is to use enzymes to generate DNA from RNA through reverse transcription reaction, and then use DNA as a template for amplification. At this time, more detectable fragments will be obtained.

At present, the rapid nucleic acid detection kits that use the fluorescent quantitative PCR method in China mainly have the advantages of high early diagnosis sensitivity, strong specificity, fast detection speed, and high popularity. For example, the new coronavirus nucleic acid rapid detection kit launched by Aisen Biotechnology Company, this new coronavirus nucleic acid rapid detection kit has obvious advantages of convenience, high sensitivity, strong specificity, and fast detection time, which greatly improves the new coronavirus Inspection efficiency can be widely used in clinical, CDC, and customs inspection and quarantine.