Introduction of Magnetic Bead Method for Nucleic Acid Extraction Kit

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There are many types of nucleic acid extraction kits，such as bacterial genomic DNA extraction kit ,whole blood genomic DNA nucleic acid extraction kit and etc. Today, we will mainly introduce the principle, use method and specific steps of the magnetic bead method nucleic acid extraction kit.

  1. What is nucleic acid

Nucleic acids are widely present in all animals, plant cells, and microorganisms. Nucleic acids in organisms often combine with proteins to form nucleoproteins.

According to different chemical composition, nucleic acid can be divided into ribonucleic acid, referred to as RNA and deoxyribonucleic acid, referred to as DNA. DNA is the main material basis for storing, copying and transmitting genetic information. RNA plays an important role in the process of protein synthesis, among which transfer ribonucleic acid, tRNA for short. Plays the role of carrying and transferring activated amino acids; messenger ribonucleic acid, referred to as mRNA, is the template for protein synthesis. Ribosomal ribonucleic acid, rRNA for short, is the main place where cells synthesize proteins.

  1. What is the magnetic bead method nucleic acid extraction kit

The magnetic bead method nucleic acid extraction kit uses a unique separation of magnetic beads and a buffer system to separate and purify high-quality genomic DNA from the sample. Special coated magnetic beads have a strong affinity for the target DNA under certain conditions. , And when the conditions change, the magnetic beads release the adsorbed DNA, which can achieve the purpose of rapid separation and purification of DNA. The whole process does not involve toxic reagents, it is safe, convenient, and the extracted DNA is of high purity. The genomic DNA (OD260-0D320/(0D280-0D320) purified by this kit is between 1.7 and 2.0, which can be applied to various downstream molecular biology experiments.

The magnetic bead method nucleic acid extraction kit uses a unique separation of magnetic beads and a buffer system to separate and purify high-quality genomic DNA from the sample. Special coated magnetic beads have a strong affinity for the target DNA under certain conditions. , And when the conditions change, the magnetic beads release the adsorbed DNA, which can achieve the purpose of rapid separation and purification of DNA. The whole process does not involve toxic reagents, it is safe, convenient, and the extracted DNA is of high purity. The genomic DNA (OD260-0D320/(0D280-0D320) purified by this kit is between 1.7 and 2.0, which can be applied to various downstream molecular biology experiments.

Accounting extraction kit: DNA extraction kit, RNA extraction kit

Composition: Lysis solution, binding solution, washing solution, eluent, biomagnetic beads, instructions

  1. Use steps of magnetic bead method nucleic acid extraction kit:

  1. Add Buffer C1, humic late adsorbent C2 and glass beads to the soil sample and vortex.
  2. Add BufferC3 to lyse the cells
  3. Add Buffer C4 to deform and precipitate protein
  4. Gaoxin removes impurities such as large humic acid
  5. Add BufferC 5. Ethanol to adjust the binding conditions
  6. Transfer to a separation column and centrifuge to adsorb DNA
  7. Add washing liquid to wash and remove impurities such as salt
  8. Centrifuge for 1min to spin dry the base shield
  9. Add TE and centrifuge for 1 min to elute DNA
  10. High-quality DNA

The above is the relevant introduction of the magnetic bead method nucleic acid extraction kit, including the classification, composition, and specific use method steps of the nucleic acid extraction kit.