What are the main components of the nucleic acid extraction kit?
Nucleic Acid Extraction Kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in nucleic acid extraction and purification technology. With the rapid development of genetic diagnosis, genetically modified food detection, personalized medicine, etc., the current nucleic acid extraction technology can no longer meet the needs of today’s biotechnology, and there is an urgent need for a high-throughput and automated nucleic acid extraction method. In this context, a nucleic acid extraction kit (magnetic bead method) came into being. Next, the editor of Aisen will introduce to you what are the main components of the nucleic acid extraction kit.
1, sodium lauryl sulfate
Sodium Lauryl Sulfate is an organic matter, white or light yellow powder, soluble in water, insensitive to alkali and hard water. It has decontamination, emulsification and excellent foaming power. It is an anionic surfactant that is slightly toxic to the human body, and its biodegradability is >90%.
It can break the hydrogen bonds in and between molecules, unfold the molecules, destroy the secondary and tertiary structure of protein molecules, denature the protein, and destroy the binding of protein and nucleic acid at high temperature, and promote the release of nucleic acid.
2, ethyl phenyl polyethylene glycol
Ethyl Phenyl Polyethylene Glycol is abbreviated as NP-40, which is a very mild non-ionic detergent, often used in cell lysis, protein treatment, histochemical treatment, hybrid washing, etc. 1% concentration can basically destroy the cell membrane, but the damage to the nuclear membrane is weak.
3, weak cation chelating resin
Weak cation chelating resin is a chemical chelating resin composed of styrene and divinylbenzene copolymer, containing pairs of iminodiacetate ions, which can chelate multivalent ions, especially for high-valent metal ions. Very high affinity and chelation. Under the conditions of low ionic strength, alkalinity and boiling, the cell membrane can be ruptured and the protein can be denatured. Chelex particles are removed by centrifugation, and the bound material is separated from the DNA.
4, proteinase K
is a powerful proteolytic enzyme isolated from Candida albicans. It has a high specific activity and is a key reagent for DNA extraction. In DNA extraction, the main function is to enzymatically decompose histones bound to nucleic acids, so that the DNA is freed in the solution.
5, polyethylene glycol octyl phenyl ether
Polyethylene glycol octyl phenyl ether is abbreviated as Triton X-100. It is a non-ionic surfactant. It does not dissociate in water, has high stability in solution, and is not easily affected by strong electrolyte inorganic salts. Combines with lipids such as phospholipids in biological membranes to form soluble complexes; the hydrophobic end can also combine with the hydrophobic regions of membrane proteins to form complexes and dissolve in solution.
6, guanidine isothiocyanate
Guanidine isothiocyanate is a powerful protein denaturant, which can quickly dissolve protein and cause cell structure to be broken. Nucleoprotein is quickly separated from nucleic acid due to the destruction and disappearance of its secondary structure.
7, ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic acid is an organic compound, which is a white powder under normal temperature and pressure. It is a chelating agent that can bind to divalent metal ions. Since most nucleases and some proteases require divalent metal ions, they are often used as inhibitors of nucleases and proteases; they can also be used to remove the inhibitory effect of heavy metal ions on enzymes.
The above is the introduction to the main components of the nucleic acid extraction kit. The nucleic acid extraction kit uses magnetic beads to adsorb DNA/RNA to achieve the purpose of rapid DNA/RNA purification, and the above-mentioned chemical components play an important role in this process. The magnetic bead method nucleic acid extraction kit designed and produced by Aisen Biotechnology is reliable in quality, convenient to use and excellent in effect. Customers in need are welcome to call for consultation.
