African Swine Fever Virus Extraction
African Swine fever (ASF) is an infectious disease caused by African Swine fever virus (ASFV, double-stranded DNA virus, 170-190kb) infecting pigs. It is a type of animal epidemic that our country focuses on preventing , The mortality rate is nearly 100%.
There is currently no vaccine available for ASF prevention. Although ASF does not infect humans, it is a devastating blow to the pig industry. The mortality rate of pigs up to 100%. China is a big pig-raising country, and the Chinese people consume extremely large amounts of pork, amounting to 1.6 trillion yuan, driving other industries more than 3 trillion yuan.
The control problem has a great impact on the pig industry and even the national economy and people’s livelihood.
PCR is currently the most commonly used method for ASFV detection in laboratories and plays an important role in the early detection of ASFV. PCR has been successfully applied to the detection of ASFV genome in pig samples (blood, organs, etc.) and ticks. Viral DNA fragments are amplified by PCR to obtain a sufficient amount for detection, and PCR can quickly become ASF detection within a few hours. Compared with ELISA and direct fluorescent antibody test (FAT), PCR has higher sensitivity and specificity, can detect very low levels of ASFV, and can quickly detect viral nucleic acid in the early stage of pig infection with ASFV.
1. Sample Collection:
Oral fluid / whole blood / serum / disease tissue;
2. Sample preparation:
Extraction and purification of high purity nucleic acid
Nucleic acid extraction instrument and nucleic acid extraction kit were used to obtain high-purity viral DNA for qPCR detection;
3. Rapid and accurate detection:
Preparation, DNA amplification, etc
QPCR instrument + African classical swine fever virus qPCR detection reagent, rapid and accurate detection of ASFV diagnosis results.
Integrated Solution of Nucleic Acid Extraction
* OEM services can be provided according to customer needs
Adaptation Reagent Model
Model: ASCEND HERO 32
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: ASCEND HERO 96
Adaptation reagent model:
0201006(96T) 0201010(48T)
Model: TianLong GeneRotex 96
Adaptation reagent model:
0201055(96T)
Model: TIANLONG NP968-S/NP968-C
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: BIOER GenePure Pro
Adaptation reagent model:
0201242(64T) 0201245(32T)
Model: BioPerfectus SSNP2000A;
SSNP3000A/SSNP-9600A
Adaptation reagent model:
0201032(64T)
Model: AllSheng Auto-Pure 96
Adaptation reagent model:
0201052(96T) 0201053(48T)
Model: Allsheng Auto-Pure 32A
Adaptation reagent model:
0201215(64T)
Model: 3DBiopharm ANDIS 350:32
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: HEMA E96-II
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: Biocomma M32
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: Thorme Kingfisher Flex
Adaptation reagent model:
0201052(96T) 0201053(48T) 0201211(96T) 0201232(24T) 0201204(48T)
Model: Zybio EXM6000
Adaptation reagent model:
0201052(96T) 0201053(48T)
Model: Zybio EXM 3000
Adaptation reagent model:
0201234(64T)
Model: TANbead smart labassit E13200
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: MOLARRAY MA-32T
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: BIOBASE BK-H32
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: Da An Gene Smart32
Adaptation reagent model:
0201108(64T)
Model: JIACHEN HBH32
Adaptation reagent model:
0201016(64T) 0201017(32T) 0201018(20T) 0201001(64T) 0201021(32T) 0201026(64T)
Model: BIAOBASE BNP96
Adaptation reagent model:
0201052(96T) 0201053(48T)