African Swine Fever Virus Extraction

African Swine fever (ASF) is an infectious disease caused by African Swine fever virus (ASFV, double-stranded DNA virus, 170-190kb) infecting pigs. It is a type of animal epidemic that our country focuses on preventing , The mortality rate is nearly 100%.

There is currently no vaccine available for ASF prevention. Although ASF does not infect humans, it is a devastating blow to the pig industry. The mortality rate of pigs up to 100%. China is a big pig-raising country, and the Chinese people consume extremely large amounts of pork, amounting to 1.6 trillion yuan, driving other industries more than 3 trillion yuan.

The control problem has a great impact on the pig industry and even the national economy and people’s livelihood.

PCR is currently the most commonly used method for ASFV detection in laboratories and plays an important role in the early detection of ASFV. PCR has been successfully applied to the detection of ASFV genome in pig samples (blood, organs, etc.) and ticks. Viral DNA fragments are amplified by PCR to obtain a sufficient amount for detection, and PCR can quickly become ASF detection within a few hours. Compared with ELISA and direct fluorescent antibody test (FAT), PCR has higher sensitivity and specificity, can detect very low levels of ASFV, and can quickly detect viral nucleic acid in the early stage of pig infection with ASFV.

1. Sample Collection:

Oral fluid / whole blood / serum / disease tissue;

2. Sample preparation:

Extraction and purification of high purity nucleic acid

Nucleic acid extraction instrument and nucleic acid extraction kit were used to obtain high-purity viral DNA for qPCR detection;

3. Rapid and accurate detection:

Preparation, DNA amplification, etc

QPCR instrument + African classical swine fever virus qPCR detection reagent, rapid and accurate detection of ASFV diagnosis results.

Integrated Solution of Nucleic Acid Extraction

* OEM services can be provided according to customer needs

Adaptation Reagent Model

ASCEND HERO 32Model: ASCEND HERO 32

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

TianLong GeneRotex 96Model: ASCEND HERO 96

Adaptation reagent model:

0201006(96T)  0201010(48T)

TianLong GeneRotex 96Model: TianLong GeneRotex 96

Adaptation reagent model:

0201055(96T)

TIANLONG NP968-S/NP968-CModel: TIANLONG NP968-S/NP968-C

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

BIOER GenePure ProModel: BIOER GenePure Pro

Adaptation reagent model:

0201242(64T)  0201245(32T)

BioPerfectus SSNP2000A;SSNP3000A/SSNP-9600AModel: BioPerfectus SSNP2000A;

SSNP3000A/SSNP-9600A

Adaptation reagent model:

0201032(64T)

AllSheng Auto-Pure 96Model: AllSheng Auto-Pure 96

Adaptation reagent model:

0201052(96T)  0201053(48T)

Allsheng Auto-Pure 32AModel: Allsheng Auto-Pure 32A

Adaptation reagent model:

0201215(64T)

3DBiopharm ANDIS 350:32Model: 3DBiopharm ANDIS 350:32

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

HEMA E96-IIModel: HEMA E96-II

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

Biocomma M32Model: Biocomma M32

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

Thorme Kingfisher FlexModel: Thorme Kingfisher Flex

Adaptation reagent model:

0201052(96T)  0201053(48T)  0201211(96T) 0201232(24T)  0201204(48T)

 Zybio EXM6000Model:  Zybio EXM6000

Adaptation reagent model:

0201052(96T)  0201053(48T)

Zybio EXM 3000Model: Zybio EXM 3000

Adaptation reagent model:

0201234(64T)

TANbead smart labassit E13200Model: TANbead smart labassit E13200

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

MOLARRAY MA-32TModel: MOLARRAY MA-32T

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

BIOBASE BK-H32Model: BIOBASE BK-H32

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

Da An Gene Smart32Model: Da An Gene Smart32

Adaptation reagent model:

0201108(64T)

JIACHEN HBH32Model: JIACHEN HBH32

Adaptation reagent model:

0201016(64T)  0201017(32T)  0201018(20T) 0201001(64T)  0201021(32T)  0201026(64T)

BIAOBASE BNP96Model: BIAOBASE BNP96

Adaptation reagent model:

0201052(96T)  0201053(48T)

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